Scientific innovation


Clinical Studies – DePaola

Clinical Studies – DePaola

Chemotherapeutic inhibition of supragingival dental plaque and gingivitis development4

- DePaola LG, Overholser CD, Meiller TF, Minah GE, Niehaus C. J Clin Periodontol. 1989;16:311-315.


Determine the efficacy of a mouthrinse used as a supplement to regular oral hygiene measures on supragingival dental plaque and gingivitis (N=107).


Double-blind, controlled clinical study of healthy male and female subjects (aged 18-60 years) with preexisting plaque and gingivitis but without evidence of periodontitis.

DePaola study

Subjects were randomized to either:

LISTERINE® Antiseptic OR 5% hydroalcohol solution

Following a complete prophylaxis on Day 1, subjects were instructed to:

Rinse with 20 mL of their assigned rinse for 30 seconds, 2x daily 6 months*

Plaque levels, gingivitis, and extrinsic tooth stain were assessed at:

  • Baseline
  • Month 3
  • Month 6


LISTERINE® Antiseptic inhibited the accumulation of supragingival dental plaque by 34.4% (P<0.001) and the development of gingivitis by 33.7% (P<0.001) at 6 months compared to hydroalcohol control.

DePaola study results


When used as a supplement to normal oral hygiene following a prophylaxis, LISTERINE® Antiseptic significantly reduced plaque and gingivitis over a six-month period, without an increase in extrinsic tooth stain.

* Subjects followed their usual oral hygiene and dietary habits.