Scientific innovation


Clinical Studies - Overholser

Clinical Studies - Overholser

Comparative effects of 2 chemotherapeutic mouthrinses on the development of supragingival dental plaque and gingivitis41, 42

- Overholser CD, Meiller TF, DePaola LG, Minah GE, Niehaus C. J Clin Periodontol. 1990;17:575-579


Determine the efficacy of 2 mouthrinses — LISTERINE® Antiseptic and Peridex® — used as supplements to regular oral hygiene measures in reducing supragingival dental plaque and gingivitis (N=124).


Double-blind, controlled, parallel design clinical study of healthy male and female subjects (aged ≥21 years) with preexisting plaque and gingivitis, but without evidence of periodontitis.

Overholser study

Subjects were randomized to one of three mouthrinse groups:

  • LISTERINE® Antiseptic
  • OR
  • PeridexTM
  • OR
  • HYdroalcohol (5%) solution

Following a complete prophylaxis on Day 1, subjects were instruted to:

Rinse with their assigned rinse for 30 seconds, 2x daily (20 mL for LISTERINE® Antiseptic and hydroalcohol groups; 15 mL for PeridexTM group)*. 6 month period

Plaque, gingivitis, bleeding, calculus, and extrinsic staining assessments were conducted at:

  • Baseline
  • Month 3
  • Month 6


At 6 months, gingivitis development was inhibited by 35.9% in the LISTERINE® Antiseptic group and by 30.5% in the Peridex™ group, when compared to the hydroalcohol control (P<0.001).

At 6 months, gingivitis development—in posterior regions—was inhibited by 33.2% and 34.4% in the LISTERINE® Antiseptic group and by 26.6% and 33.8% in the Peridex™ group, when compared to the hydroalcohol control (P<0.001).42

Data from Overholser study


LISTERINE® Antiseptic produces gingivitis reductions comparable to Peridex™ at 6 months (including gingivitis in posterior regions).

*During the study, subjects followed their usual oral hygiene and dietary habits.